Transgenic Rescue of the LARGEmyd Mouse: A LARGE Therapeutic Window?
Fig 8
Expression of endogenous LARGE increases during myogenesis in both normal and dystrophic cells.
qPCR analysis of cDNA prepared from WT (H2K2B4) or mdx (H2KSF1) cell cultures at the times indicated using primers for endogenous LARGE (upper panel), and for myogenic transcription factors MyoD and MEF2C (lower panels). Expression levels are normalised (using Csnk2a2 + Ap3d1) and shown as fold increase over time-zero (proliferating) levels (Means + S.E.M, N = 3).
