Tendon Reattachment to Bone in an Ovine Tendon Defect Model of Retraction Using Allogenic and Xenogenic Demineralised Bone Matrix Incorporated with Mesenchymal Stem Cells
Fig 1
A. Ovine patellar-tendon defect. B. Osteotomised tibial tuberosity with two suture anchors in situ. The defect was repaired with allograft in five sheep and with xenograft in the remaining five sheep. An interlocking Krakow suture technique was used to secure the DBM strip to the remaining proximal patellar tendon using a #2 FiberWire (Fig 1C). The distal end of the DBM strip overlying the tibial tuberosity was secured to the prepared flat surface using the two anchors and its margins sutured to the surrounding tissue to ensure consistent and complete contact with the bony footprint. The dimensions of the DBM strip were adjusted to match the length and width of the host patellar tendon prior to repair: averaged 20 mm wide and 100 mm long. The surgical wound was closed in layers using absorbable Vicryl sutures. The sheep were allowed to move freely postoperatively without restraint. Animals received intravenous Buprenorphine at a dose of 0.006–0.01 mg/kg for a maximum of four days after surgery. Digital lateral X-ray radiographs of both hind limbs were taken at 12 weeks. Fig 1C. Ovine patellar tendon defect repaired with allogenic DBM.
