Retinol Promotes In Vitro Growth of Proximal Colon Organoids through a Retinoic Acid-Independent Mechanism
Fig 1
Expression of genes involved in ROL-RA pathway in colonic epithelia.
(A) Crypts isolated from the entire length of the colon were cultured for 6 days. Organoids were then dissociated into single cells and further cultured in the presence of vehicle alone, or indicated concentrations of ROL. Phase-contrast images were acquired on Day 5 of culture. Representative images of three independent experiments are shown. Scale bar, 100 μm. (B) Total RNA was extracted from the whole colon or its epithelial compartment isolated as crypts. Semi-quantitative PCR was performed for the indicated genes. Representative data are shown for three independent experiments.
