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Identification of Unanticipated and Novel N-Acyl L-Homoserine Lactones (AHLs) Using a Sensitive Non-Targeted LC-MS/MS Method

Fig 4

3-oxo-C7-HL detection in P. stewartii.

(A) Fragmentation spectra when bacteria are grown in medium with no labeled components, medium with 100% uniformly labeled glucose (¹³C labeling), and medium with labeled ammonium (¹⁵N labeling). Characteristic lactone ring fragments are highlighted in blue and parent ion is highlighted in red. (B) Structure of 3-oxo-C7-HL and its ¹²C, ¹³C, and ¹⁵N masses. (C) Structures and masses of fragments of 3-oxo-C7-HL in non-labeled, ¹³C-labeled, and ¹⁵N-labeled media. Similar results were observed for E. carotovora.

doi: https://doi.org/10.1371/journal.pone.0163469.g004