Identification of Unanticipated and Novel N-Acyl L-Homoserine Lactones (AHLs) Using a Sensitive Non-Targeted LC-MS/MS Method
Fig 5
(A) AHL standards were added at 1 μM concentration to exponentially growing cultures and AHL degradation was measured after 24 hours. The amount of AHL degradation at 24 hours is shown as: +++, >90% degradation; ++ 60–90% degradation; +, 30–60% degradation; -, less than 30% degradation. (B) Dynamic AHL degradation in selected bacteria. AHLs (10 μM each) were added to exponentially growing cultures and AHL degradation was monitored over time. Data represents the average of three biological replicates.