Heparanase Overexpression Reduces Hepcidin Expression, Affects Iron Homeostasis and Alters the Response to Inflammation
Fig 3
Treatment with heparin and IL6 of HepG2 clones overexpressing heparanase.
(A) The two HepG2 clones overexpressing HPA (HPA3 and HPA6) and control (MOCK) cells were treated with 0.12 μg/mL of RO-82 heparin for 6 h and hepcidin mRNA evaluated. (B) The clones and control cells were treated with 50 ng/mL IL6 for 3 h and analyzed for mRNA level of Socs3 and (C) hepcidin in relation to Hprt1. The values are expressed as fold change of their respective untreated controls (-). (D) Western blot of pSMAD5, total SMAD5, pSTAT3, total STAT3 and of ACTIN as housekeeping.
