Heparanase Overexpression Reduces Hepcidin Expression, Affects Iron Homeostasis and Alters the Response to Inflammation
Fig 8
Transgenic mice overexpressing Heparanase are more sensitive to inflammatory stimuli.
WT and TG-HPA mice were treated with a single dose of LPS (1 mg/Kg) IP and sacrified after 6 h. (A) Analysis of Socs3 mRNA, (B) CRP mRNA and (C) HAMP1 mRNA in the liver of WT and TG-HPA mice in relation to Hprt1, before and after the treatment. The values are expressed as fold change of wild type mice. (D) Quantification of serum Hepcidin by SELDI-TOF. (E) Western blot of pSMAD5, total SMAD5, pSTAT3, total STAT3 and of GAPDH as housekeeping.
