Characterization of Triticum aestivum Abscisic Acid Receptors and a Possible Role for These in Mediating Fusairum Head Blight Susceptibility in Wheat
Fig 4
Effect of the VIGS knockdown of Wheat Ta_PYL4AS_A on F. graminearum infection of T. aestivum cv. โFielderโ.
A) Relative expression of Ta_PYL4AS_A in various VIGS treated wheat plants. Quantification of the gene expression of Ta_PYL4AS_A was performed 12 days post BSMV:Ta_PYL4AS_A application (+/- F. graminearum (Fg) application 7 days after the BMSV rub), compared to controls treated with BMSV:GFP, with Ta_actin used as an endogenous control. Uninfected plants, black bars; Fg infected plants, white bars. The expression level of the BMSV:GFP control was set to a value of 1 (n = 3). BSMV treated plants were treated with F. graminearum spores 7 days after the BMSV rub and assessed for F. graminearum infection in the B) spikelets and C) rachis at days 3, 5, 7 9 and 13 postโF. graminearum infection in BSMV:GFP (black bars) and BSMV:Ta_PYL4AS_A (white bars). Data were tested using 2-way ANOVA, and for each day separately using a t-test. (n = 9). D) Accumulation of DON in BSMV:Ta_PYL4AS_A (black bars) and BSMV:GFP (white bars) treated wheat heads. Samples were analyzed by LC/MS at 5 and 13 days post F. graminearum infection (n = 3). For all panels, error bars indicate standard error of the mean. Significant differences between samples are indicated with asterisks, (* p < 0.05, ** p < 0.01, *** p < 0.001).
