Defective Autophagy, Mitochondrial Clearance and Lipophagy in Niemann-Pick Type B Lymphocytes
Fig 5
Evaluation of intracellular lipid content by Nile Red (NR).
(A) Single confocal optical sections (~0.8 Ξm thickness) showing overlay of yellow (FL2) and red (FL3) NR fluorescence from all experimental conditions in tWT and tNP cells. Bars: 10 Ξm. (B) Statistical histogram of FL2 MFI variation of intracellular NR in tWT and tNP cells for each experimental condition. Mean values were converted to arbitrary units (A.U.) setting control of wild-type cells as 100. Each value is expressed as a relative mean Âą SD (Results from n âĨ 3 independent experiments); **P < 0.01 vs respective control.
