Denervation-Induced Activation of the Standard Proteasome and Immunoproteasome
Fig 4
Protein content of standard catalytic and immunoproteasome subunits in WT and L7M1 mice with denervation.
Results of densitometry are presented as the fold change compared to WT Day 0 for each respective protein. (A-C) Standard catalytic subunits. Each panel contains results of two-way ANOVA that were used to detect the effect of strain (WT vs. L7M1) and time (Day 0, 7, and 14) for (A) β1, (B) β5, and (C) β2 (# indicates significant effect of strain/time/S x T interactions, p < .05). When an effect of interaction was detected, a Fisher’s LSD post-hoc test was followed to compare differences between strains at each time point (* indicates L7M1 is significant different from WT at that given time point, p ≤ .05). Statistical comparison showed a significant denervation (time) effect in all the proteins above (p < .001). No strain effect was detected in any of these proteins. (A) β1 was significantly higher at day 7 in the WT compared to L7M1 (S x T, p = .01, LSD post-hoc test: p = .003). (B) β5 was significantly higher at day 7 in the WT compared to L7M1 (S x T, p = .01, LSD post-hoc test: p = .002). (D-F) Immunoproteasome subunits. For (D) LMP7 content and (E) MECL-1 content, the one-way ANOVA statistical comparison showed a significant denervation (time) effect in LMP7 and MECL-1 content (p < .001). # indicates significant time effect; ¶ indicates significant difference comparing to the 0d. (F) For LMP2 content: The two-way ANOVA result is shown in this panel (# indicates significant effect of strain/time, p < .05). Statistical comparison showed a significant denervation (time) effect (p < .001) and strain effect (p = .003) in LMP2 content. Values are mean ± SE. Sample size per group: n = 4–10. Representative Western blot for each protein is presented in Fig 1.
