Denervation-Induced Activation of the Standard Proteasome and Immunoproteasome
Fig 5
Proteasome enzymatic activities in WT and L7M1 mice with denervation.
Results are presented as the fold change compared to WT Day 0 for proteasome enzymatic activity in each experimental group. Two-way ANOVA was used to detect the effect of strain (WT vs. L7M1) and time (Day 0, 7, and 14) for (A) Chymotrypsin- like (CT-L) activity, (B) Trypsin- like (T-L), and (C) Caspase- like (C-L) activity (# indicates significant effect of strain/time/S x T interactions, p < .05). When an effect of interaction was detected, a Fisher’s LSD post-hoc test was performed to compare differences between strains at each time point (* indicates L7M1 is significant different from WT at that given time point, p ≤ .05). Statistical comparison showed a significant denervation (time) effect in all proteasome activities (p < .001). No strain effect was detected in either CT-L or T-L activities; whereas the C-L activity showed significant strain difference (p = .01). (A) CT-L activity was significantly higher in the WT at day 7 compared to L7M1 (S x T, p = .04, LSD post-hoc test: p = .04). (B) T-L activity was significantly lower in the WT at day 14 compared to L7M1 (S x T, p = .02, LSD post-hoc test: p = .03). Values are mean ± SE. Sample size per group: n = 7–10.
