System-Wide Adaptations of Desulfovibrio alaskensis G20 to Phosphate-Limited Conditions
Fig 2
Transmission electron and epifluorescence micrographs of G20 from phosphate-replete and phosphate-limited cultures.
(A) Blue fluorescence of DAPI-stained G20 cells not limited by phosphorus. (B) Green fluorescence of DAPI-stained G20 not limited by phosphorus indicates the presence of polyphosphate. (C) Blue fluorescence of elongated, DAPI-stained G20 cells limited by phosphorus. (D) Green fluorescence of DAPI-stained G20 cell limited by phosphate is not detectable due to the absence of polyphosphate granules. Panels (A)-(D) are shown on the same scale. (E) Cells grown at 27°C in MOLS4 with 200 μM initial phosphate. (F) Cells grown at 27°C in MOLS4 with 2 μM initial phosphate. Panels (E) and (F) are shown at the same scale shown in (F). Insets in (E) and (F) show enlarged areas outlined by the black rectangles. Black arrows point to the inner and outer membranes, p indicates the periplasm, Pi marks polyphosphate granules, bm labels a representative area filled with bright material, dg marks a small dark granule.
