Fibroblast activation protein is dispensable in the anti-influenza immune response in mice
Fig 1
Effects of genetic deficiency of FAP on the numbers and phenotype of leukocytes at steady state.
Organs were harvested from FAP knockout (gko; closed symbols) and wildtype (WT; open symbols) C57BL/6 mice. A. The numbers of thymic CD4-CD8- double negative (DN), CD4+CD8+ double positive (DP) CD4+CD8- and CD8+CD4- single positive (SP) populations. B. The numbers of splenic CD4+ and CD8+ T cells. C. The percentages of CD44- naïve, CD25+ regulatory and CD44hi activated/ memory CD4+ T cells in the thymus and the spleen. D. The percentages of CD8+ T cell subsets gated according to their expression of CD44 and CD62L in the thymus and the spleen. E. The numbers of dendritic cells (DC) in the spleen, peripheral LN (pLN), mesenteric LN (mLN) and thymus in FAP knockout and WT mice at steady state. F. The geometric mean fluorescence index (MFI) of surface expression of CD86 (top) and B7H1/PD-L1 (bottom) on DC in the spleen, pLN, mLN and thymus. G. The numbers of splenic CD11b+Ly6G+ neutrophils. H. The numbers of splenic CD19+ B cells. Each symbol represents one mouse and the bar represents the mean. Except for part F, results were pooled from two independent experiments of n = 4 mice each. For F, results from one of three experiments (n = 4 mice each) are shown. Statistical significance was tested using the Mann–Whitney test. * p<0.05, ** p<0.01.
