Fibroblast activation protein is dispensable in the anti-influenza immune response in mice
Fig 3
T and B cell responses to influenza in FAP knockout (gko) and wildtype (WT) mice.
A & B. FAP knockout (gko; closed symbols) and wildtype (WT; open symbols) mice received intravenous transfer of donor OT-1 cells and on the next day were intranasally inoculated with 100 pfu PR/8-OVA influenza virus. Organs were obtained on day 7 post-infection. A. The numbers of donor cells recovered from recipient lungs and mediastinal lymph nodes (mdLN) on day 7 post-infection. B. Cells harvested from the lungs and mdLN were restimulated in vitro in the presence of SIINFEKL peptide and the production of IL-2 (top) and IFN-γ (bottom) by donor OT-I cells was quantified by flow cytometry. The percentage of positive events in stained samples and fluorescence minus one (FMO) negative controls are shown. Each data point represents an individual mouse and the bar represents the mean. Results from one of three experiments are shown. Statistical significance was tested using the Mann–Whitney test. C. FAP knockout and WT mice were intranasally infected with 50 pfu PR/8 virus and sera were harvested on day 21 post-infection. Neutralising anti-influenza antibody titres in the sera were measured using haemagglutination inhibition (HI) assay. Each data point represents an individual mouse and the bar represents the mean. Statistical significance was tested using Kruskal-Wallis test with Dunn’s multiple comparison test. * p<0.05, *** p<0.001, ns not significant.
