Effect of intravenous immunoglobulin on the function of Treg cells derived from immunosuppressed mice with Pseudomonas aeruginosa pneumonia
Fig 5
Serum concentrations of active TGF-β in each group at 4, 8 and 24h.
Six groups were studied: Control group, IS group, PA group, IS + PA group, PA + IVIG group and IS + PA + IVIG group. Immunosuppression was induced on day -5, -3 and -1 by intraperitoneal injection of CYP, PA pneumonia was induced on day 0 and IVIG mediated treatment models were immediately established following PA intervention on day 0. Serum samples were heated in a water bath at 80°C for 8 min and cooled for 5 min. The serum concentration of active TGF-β was tested by ELISA kit and was determined by comparison with a standard curve. Serum concentrations of active TGF-β in six groups at 4, 8 and 24h were shown.
