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A comparative study of the effect of the dose and exposure duration of anabolic androgenic steroids on behavior, cholinergic regulation, and oxidative stress in rats

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Experimental Protocols: Protocol I: Intramuscular injection of vehicle (olive oil, 0.2 ml), stanazolol (ST, 5 mg/kg), or boldenone (BOL, 5 mg/kg) once a week for 4 weeks. Protocol II: Intramuscular injection of vehicle (olive oil, 0.2 ml), stanazolol (ST, 2.5 mg/kg), or boldenone (BOL, 2.5 mg/kg) once a week for 8 weeks. Protocol III: Intramuscular injection of vehicle (olive oil, 0.2 ml), stanazolol (ST, 1.25 mg/kg) or boldenone (BOL, 1.25 mg/kg) once a week for 12 weeks. Day 1: elevated plus maze task (EPM). Day 2: object recognition task, habituation. Day 3: object recognition task, training. Day 4: object recognition task, testing. Day 5: agonist behavior (resident–intruder paradigm), (n = 10).

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doi: https://doi.org/10.1371/journal.pone.0177623.g001