http://orcid.org/0000-0001-9617-0300
Figures
Abstract
In this study, we designed a microcosm experiment to explore the composition of the bacterial community in the rhizosphere of maize and bulk soil by sequencing the V3-V4 region of the 16S rRNA gene on the Illumina system. 978–1239 OTUs (cut off level of 3%) were found in rhizosphere and bulk soil samples. Rhizosphere shared features with the bulk soil, such as predominance of Acidobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes and TM7. At genus level, many of the dominant rhizosphere genera (Chitinophaga, Nitrospira, Flavobacterium, etc.) displayed different patterns of temporal changes in the rhizosphere as opposed to the bulk soil, showing rhizosphere has more impact on soil microorganisms. Besides, we found that significant growth-related dynamic changes in bacterial community structure were mainly associated with phylum Bacteroidetes, Proteobacteria and Actinobacteria (mainly genera Burkholderia, Flavisolibacter and Pseudomonas), indicating that different growth stages affected the bacterial community composition in maize soil. Furthermore, some unique genera in especial Plant-Growth Promoting Rhizobacteria (PGPR) such as Nonomuraea, Thiobacillus and Bradyrhizobium etc., which were beneficial for the plant growth appeared to be more abundant in the rhizosphere than bulk soil, indicating that the selectivity of root to rhizosphere microbial is an important mechanism leading to the differences in the bacteria community structure between rhizosphere and bulk soil.
Citation: Yang Y, Wang N, Guo X, Zhang Y, Ye B (2017) Comparative analysis of bacterial community structure in the rhizosphere of maize by high-throughput pyrosequencing. PLoS ONE 12(5): e0178425. https://doi.org/10.1371/journal.pone.0178425
Editor: Yi Luo, Nankai University, CHINA
Received: January 3, 2017; Accepted: May 12, 2017; Published: May 25, 2017
This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Data Availability: All relevant data are within the paper.
Funding: This study was financially support by the Natural Science Foundation of China (No. 21507037), Commonwealth and Environmental Protection Project granted by the MEP (No. 201309031), as well as the Fundamental Research Funds for NIES in 2016 “Source Apportionment Research of antibiotics pollution in typical drinking water resource”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Soil microorganisms are not only an important part of soil, but also the main driver of soil nutrient cycling [1]. Soil microorganisms play critical roles in regulating soil fertility, plant health, and the cycling of carbon, nitrogen, and other nutrients.
Plants exert selective pressure on the structural and functional diversity of microbial populations through the root exudation, and in relation to soil properties, plant species, growth stage, and many other stress factors [2]. Rhizosphere was defined as a “hot spot”, rhizosphere-associated microbes are more active in this zone and possess diverse metabolic capabilities and play a crucial role in plant health and soil fertility [3]. Some bacteria that colonize the roots are plant growth-promoting rhizobacteria (PGRP), which are more closely associated with plant growth than those in bulk soil. Among them, some soil microbial communities which inhabit in rhizosphere, may restrict or inhibit the growth of potential pathogens by producing antibiotics, antifungal chemicals and insecticides [4]. Furthermore, these microbial also have the function to solubilize phospate and nitrogen. As study has reported, the genera Bacillus, Pseudomonas, Enterobacter, Acinetobacter, Burkholderia, Arthrobacter and Paenibacillus are the common PGPRs in the rhizosphere [5].
Nelson [6] suggested the root exudates quantity and quality-mediated changes in mineral nutrition, which is essential for rhizosphere community structure. Besides, root physiology will be influenced by plant growth stage and the quality and quantity of root exudates can also be changed; thus, these changes exert a selective pressure on root-associated bacterial at different growth stages [7]. Previously studies demonstrated that seasonal variations in the activity and relative abundance of rhizosphere microorganism communities are closely related to plant type [8–10].
It has been estimated that the microorganisms which can be cultured in laboratory conditions occupied only a small part of these populations (<1%). With the continuous advances of high-throughput pyrosequencing technologies, the research on the microbial flora in the soil has become more and more thoroughly, thus provides a possibility for comprehensive analysis of the taxonomy, phylogenetic, and functional diversity of microbial communities [5, 11–12].
Since maize is one of the most important economic crops and the microbe structure in rhizosphere plays a crucial role in plant growth, it is imperative to give a better understanding of their individual roles. Thus, in this work we have carried out a microcosm experiment, with maize as an ideal plant for investigation. The aim was to assess and add some new insight into the influence of growth stage on composition of the bacterial community in the rhizosphere and bulk soil. It was also of interest to identify dominant and sensitive soil bacteria and in the hope of contributing to provide relevant data for further research.
Materials and methods
Experiment design
The study was permitted and approved by the Ministry of Environmental Protection, China. The land accessed was not privately owned or protected. No protected species were sampled. Manure was collected from pigs at the experimental farm of Jiangsu Province Tomorrow Agriculture and Animal Husbandry Science and Technology Co., Ltd. The soil used for microcosm were paddy soil from the experimental farm of Nanjing Institute of Environment Science, MEP with 49.8 g·kg-1 organic carbon content, a pH of 6.23, an ion exchange capacity of 18.0 cmol·kg-1, 19.4% clay, 75.8% silt, and 4.8% sand. Manure and soil were mixed with an electric mixing machine at a ratio of 1:25 (w/dw), and the soil was filled into polystyrene tubes (inner diameter: 17cm, height: 60cm) that were planted with maize (Zea mays L.). The plants were kept in green house with a day/night cycle of 16:8 h. Water loss was replenished daily to keep soil moisture varied between 8.5 and 15.2% (w/dw).
Sampling and DNA extraction
Soil samples were collected after maize had grown for 14d, 35d, and 63d. The maize roots were shaken vigorously to remove loosely adhered soil which were defined as bulk soil (BK), while the soil that tightly adhered to the roots was regarded as rhizosphere (RH), soil samples were named BK-1, BK-2, BK-3, and RH-1, RH-2, RH-3, respectively. All samples transported on ice to be stored at 4°C before analysis in the lab. The total DNA extraction from rhizosphere and bulk soil samples was performed employing a PowerSoil DNA Isolation Kit (USA, MOBIO) according to the protocol. The concentration and quality of the extracted total DNA were examined by UV spectrophotometry (Nanodrop 1000, Thermo, USA) and 1% agarose gel electrophoresis.
High-throughput 16s rRNA pyrosequencing
For gene libraries construction, the bacterial 16S rRNA gene was amplified by PCR with a set of primers targeting the hypervariable V3–V4 region (about 460 bp). The V3 forward primer was 5′–ACTCCTACGGGAGGCAGCAG-3′ and V4 reverse primer was 5′- TACNVGGGTATCTAATCC-3′. The PCR procedures were as follows: 94°C for 3min as an initial denaturation, 30 cycles of 94°C for 30s, 62°C for 30s, 70°C for 45s, and 72°C for 7 min as a final extension. A total volume of reaction mixture was 50μL which consisted of 1×Pfx Amplification Buffer (Invitrogen, USA), 0.4 mM dNTP, 2 mM MgSO4, 0.4 μM each primers, 1 μL of template DNA and 2 U of Platinum® Pfx DNA Polymerase (Invitrogen, USA). The extracted DNA was subject to high-throughput pyrosequencing using TruSeq SBS Kit V3 on Illumina Hiseq 2000 (Illumina, USA). The Illumina pyrosequencing strategy was “Index 101 PE” (Paired End sequencing, 101-bp reads and 8-bp index sequence), which generates nearly equal amount of clean reads for each sample. For quality control, the raw reads containing three or more “N” or contaminated by adapter (>15 bp overlap) were removed.
Analysis of microbial community and biodiversity
After 454 pyrosequencing, the clean high-quality sequence were grouped into operational taxonomic units (OTUs) using the Quantitative Insights into Microbial Ecology (QIIME) at 97% sequence identity [13]. Representative sequences from each OTU were assigned down to the phylum and genus using software RDP MultiClassifier at 80% confidence [14]. Richness and diversity indices including operational taxonomic units (OTUs), Chao estimator and abundance-based coverage estimator (ACE), as well as rarefaction curves, were calculated using Mothur.
Results
Overall structural variance of bacterial communities
After sequencing the V3-V4 region, a total of 218905 raw reads were generated from soil samples. As shown in Table 1, after filtering the low quality reads using the QIIME' standard pipeline and trimming the primers, adapters and barcodes, a total of 35603–36388 and 35587–36492 high quality clean reads were obtained for rhizosphere and bulk soil, respectively.
For detail, 978–1239 OTUs (cut off level of 3%) were found in rhizosphere and bulk soil samples. The OTUs first increased and then decreased in all soil samples as maize growth progressed, and were consistently higher in rhizosphere than those in bulk soil.
The Shannon index yielded values ranging from 5.330 to 6.228 for rhizosphere and from 5.240 to 5.769 for bulk soil, suggesting a relatively higher diversity of bacterial sequences in the rhizosphere. The values of ACE for samples from rhizosphere were ranged from 1285.612 to 1479.729, whereas those for samples from bulk soil were 1337.056 to 1335.411. Overall, both the index of Chao, ACE, Shannon and Simpson revealed similar trends, with higher values observed for rhizosphere samples, when compared with samples from bulk soil, indicating that the higher abundance of bacterial community in rhizosphere, however, the difference among them was not significant.
Rarefaction curve was the common used method to compare species diversity in the ecosystem. The rarefaction curves of the two soil groups were also drawn in this study (Fig 1). Results found that the rarefaction curves of these samples didn’t approach the asymptote, which indicated that each sample showed highly diversified microbial communities and further sequencing would have generate more OTUs in each sample. In this study, these curves indicated that OTUs were higher in rhizosphere than in bulk soil at each sampling time, with the OTUs increased in 35d and then decreased in 63d.
Differences in bacterial community composition
The average relative abundances are presented in Fig 2, and the classified sequences were associated with 28 phyla, but only 9 were found at a relative abundance of >1%. The major phylum groups were Acidobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes and TM7, accounted for more than 90% of bacterial sequences in both rhizosphere and bulk soil.
Fig 2 exhibits the main phylum compositions of rhizosphere and bulk soil samples. Rhizosphere was mainly composed of Proteobacteria (19.3%~40.2%), Bacteroidetes (19.1%~30.8%), Firmicutes (5.4%~19.4%), Actinobacteria (6.3%~11.7%) and Acidobacteria (6.5%~15.0%). Bulk soil was mostly comprised by Proteobacteria (15.8%~38.3%), Bacteroidetes (27.2%~32.2%), Firmicutes (8.1%~16.2%), Acidobacteria (6.3%~14.1%) and Actinobacteria (5.4%~11.9%). WS3, Chlorobi, Cyanobacteria, Elusimicrobia, Nitrospirae, Planctomycetes and Tenericutes were present in most soil samples, but at relatively low abundance (Fig 2).
Many sequences could not be classified but were abundant in rhizosphere and bulk soil (1.22%~18.95%), demonstrating that soil remains a challenging reservoir of biodiversity. Moreover, the ratio of sequences in rhizosphere and bulk soil was 63d>35d>14d, indicating that bacterial diversity was modified with root activity at different sampling times.
A thorough investigation at the genus level showed an enrichment trend of predominant bacterial groups in soil samples. The relative abundance of any genus was <7% in each sample, implying high bacterial diversity in the two sample groups. As shown in Tables 2 and 3, 44 genera were obtained in all soil samples. Chitinophaga, Flavisolibacter, Nitrospira, Pseudomonas and Streptomyces which were predominantly found in the rhizosphere and bulk soil, belong to Bacteroidetes, Proteobacteria and Actinobacteria, respectively.
The relative abundance of Streptomyces, Burkholderia, Caulobacter, Devosia, Glycomyces and Ralstonia was higher in rhizosphere than bulk soil, while DA101, Dyadobacter, Niastella, Pseudomonas and Sorangium demonstrated the reverse.
The OTU abundance varied from sampling times. For example, Vrhodoplanes, Streptomyces, Nocardia, Actinomadura, Amycolatopsis, Dokdonella and Niastella significantly increased in 35d and/or 63d in bulk soil, rising by 119.01%-3549.86%. In the rhizosphere, Nocardia, Pseudoxanthomonas, SMB53, Sphingobacterium, Turicibacter, Actinomadura, Amycolatopsis and Dokdonella was elevated by 308.97%-3747.85%, compared to the last sampling time.
The abundance of Niastella and Flavisolibacter in rhizosphere was decreased in 35d and then increased in 63d, while difference occurred in bulk soil, with a continued increase from 14d to 63d. The results suggest that bacterial community composition was altered markedly to a greater extent in the rhizosphere than bulk soil, indicating that rhizosphere bacteria may respond first to environmental changes ahead of bulk soil.
Comparison on microbial genera
In this study, some unique genera in rhizosphere and bulk soil were found, respectively (Table 4). Among them, 8 special genera were obtained in rhizosphere, according to the function can be divided into: anti-bacteria (Nonomuraea), nitrogen fixing bacteria (Phenylobacterium, Inquilinus, Bradyrhizobium and Nitrosovibrio), phosphate solubilizing bacteria (Thiobacillus and Erwinia), and pathogenic bacteria (Rickettsia); while, 5 unique genera detected in bulk soil were divided into: phosphate solubilizing bacteria (Gemmatimonas), nitrogen fixing bacteria (Azospirillum and Actinomycetospora), pathogenic bacteria (Aquicella and Conexibacter). In rhizosphere, most unique genera bacteria belongs to PGPR, and the main function were phosphorus solubilizing, nitrogen fixation, and degrade cellulose, while in bulk soil belongs to PGPR and pathogenic bacteria.
Discussion
Soil is a very complex ecological system, and the soil microorganism plays an important role in the soil. High-throughput pyrosequencing is considered to be a good method to study soil microbial diversity, which can be used to carry out high quality sequencing of DNA specific sections of soil microbial communities. In this study, the diversity, composition, and relative abundance of bacterial community in rhizosphere and bulk soil were represented at different sampling times during the maize growth process, via Illumina high-throughput sequencing. The results provided insights into the effects of alterations in maize root exudates on microbial community.
After sequencing the V3-V4 region of 16S rRNA, 215903 effective sequences were obtained in total with 35983 sequences per sample on average, then clustered into 978~1167 OTUs at 97% similarity level. By comparing with the database, we found that the composition of microbial community in rhizosphere was similar but higher than bulk soil on the phylum level. However, with the growth period of maize, the relative abundance of each phylum varying in soil samples, which may be related to soil structure, nutrients, root exudates and other factors.
Proteobacteria is the predominant phylum in rhizosphere may due to their rapid growth rates and, this nutrient-rich environment was suit for the phylum Proteobacteria, or certain classes within this phylum [15]. This genus bacterium is mostly Gram-negative and many are responsible for nitrogen fixation and polycyclic aromatic hydrocarbons. In according with previous study that Acidobacteria were similar regardless of crop variety (grass or wheat) and land management practice [4]. The number of Bacteroidetes in intestinal accounted for more than half of total bacteria, thus can be widely detected in manure and manure-amended soil. Actinobacteria are widely founded in soil and water ecosystem and play a critical role in the decomposition and humus formation process [16]. Overall, these findings were in consistent with previous studies on bacterial communities in soil [17, 18]. These authors concluded that major soil phyla were comprised of Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Firmicutes and Plantcomycetes, although recognizing that their relative abundances vary with the study site.
There were several key microbial groups that displayed different temporal dynamics in rhizosphere from that in the bulk soil. In fact, many of the dominant rhizosphere genera (Chitinophaga, Nitrospira, Flavobacterium, etc.) displayed different patterns of temporal changes in the rhizosphere as opposed to the bulk soil (Tables 2 and 3), showing rhizosphere has more impact on soil microorganisms. It can be expected that the composition and chemical nature of root exudates will change over the plant growth cycle. In this study, we found that significant growth-related dynamic changes in bacterial community structure were mainly associated with phylum Bacteroidetes, Proteobacteria and Actinobacteria (mainly genera Burkholderia, Flavisolibacter and Pseudomonas). Members of Burkholderia were enriched in the rhizosphere, possibly due to their versatile abilities to utilize root metabolites, degrade aromatic compounds [19] and produce anti-microbial substances. Pseudomonas has rapid growth and is therefore good colonizers in soil. Pseudomonas can use various substrates as nutrients and survive under different stressing conditions. Also its ability to produce various compounds, such as antibiotics, polysaccharides and siderophores are crucial to its success. Li et al [20] reported that the genera Flavobacterium may be copiotrophic and fast-growth bacteria, able to exploit a transient niches for nutrition and others at the early growth stage of maize. The decline of Flavobacterium at later stages may be explained by the reduction of quantity and quality of organic carbon, or interspecies competition for resources.
There were more than 2.6×1029 live cells in soil [17], but majority of them can be found in rhizosphere. The roots of plant seem to having a stronger impact on microbial activity. Some microorganisms and some especial bacteria, which are known as Plant-Growth Promoting Rhizobacteria (PGPR) benefit plant growth and health. These bacteria can increase the availability of nutrients for the plant in the rhizosphere. Previous researches had also shown that fertilizer amount can be reduced after adding the PGPR bacteria into crops [21].
It is essential to give insight to the microbial biodiversity since the bacterial diversity and soil function are correlated. Most of the bacteria in the rhizosphere are phosphate solubilizing, nitrogen, fixing and plant disease suppressing which helps to improve the crop yield and protection. In rhizosphere, most unique genera bacteria belongs to PGPR, and the main function were phosphorus solubilizing, nitrogen fixation, and degrade cellulose, while in bulk soil belongs to PGPR and pathogenic bacteria.
These changes in the bacterial species may have a close relationship with the root exudates of maize. For example, Nitrogen fixing bacteria are very sensitive to sugar and amino acids in root exudates, thus leading to a large number of bacteria with nitrogen fixing function in the rhizosphere [22]. Besides, pH, soil structure, organic matter and nutrient levels in the rhizosphere and bulk soil were different and thus can led to more beneficial microbes dwelling in the rhizosphere region [23]. Meanwhile, rhizosphere can alter plant physiological and biochemical processes by changing the rhizosphere nutrition status and effecting plant hormone content, which makes the interaction between rhizosphere and microbes develop toward to the more conducive direction of both sides.
Conclusion
At this present study, we have analyzed the maize bacterial community in the rhizosphere and bulk soil at different growth stages. Overall, the abundance and diversity of microbial community in rhizosphere were higher than bulk soil, and related closely with maize growth stages. Besides, some growth-promoting microorganisms beneficial for the plant growth appeared to have been more abundant in the rhizosphere than bulk soil, indicating that selectivity of root to rhizosphere microbial is an important mechanism leading to the differences in the bacteria community structure between rhizosphere and bulk soil.
Author Contributions
- Conceptualization: NW YY BY.
- Data curation: YY.
- Funding acquisition: NW BY.
- Investigation: YY XG YZ.
- Methodology: YY XG.
- Project administration: YY BY.
- Resources: NW BP.
- Software: YY.
- Supervision: NW BY.
- Visualization: XG YZ.
- Writing – original draft: YY.
- Writing – review & editing: NW BY.
References
- 1. Baudoin E, Benizri E, Guckert A. Impact of artificial root exudates on the bacterial community structure in bulk soil and maize rhizosphere. Soil Bio Biochem. 2003; 35(9), 1183–1192.doi.org/10.1016/S0038-0717(03)00179-2
- 2. Gomes NCM, Heuer H, Schönfeld J, Costa R, Mendonça-Hagler L, Smalla K. Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis. Plant Soil. 2001; 232(1):167–180.
- 3. Dennis PG, Miller AJ, Hirsch PR. Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities. Fems Microbiol Ecol. 2010;72(3):313–327. pmid:20370828
- 4. Garcíasalamanca A, Molinahenares MA, Dillewijn PV, Solano J,Pizarrotobías P. Bacterial diversity in the rhizosphere of maize and the surrounding carbonate-rich bulk soil. Microb Biotechnol. 2013;6(1):36–44. pmid:22883414
- 5. Zhang X, Zhang R, Gao J, Wang X, Fan F, Ma X, et al. Thirty-one years of rice-rice-green manure rotations shape the rhizosphere microbial community and enrich beneficial bacteria. Soil Bio Biochem. 2017;104:208–217.
- 6. Nelson D R, Mele P M. Subtle changes in rhizosphere microbial community structure in response to increased boron and sodium chloride concentrations. Soil Bio Biochem. 2007; 39(1):340–351.
- 7. Houlden A, Timmswilson T.M, Day M J, Bailey M J. Influence of plant development stage on microbial community structure and activity in the rhizosphere of three field crops. 2008; 65(2):193–201. pmid:18616582
- 8. Dohrmann A B, Küting M, Jünemann S, Jaenicke S, Schlüter A, Tebbe CC. Importance of rare taxa for bacterial diversity in the rhizosphere of Bt-and conventional maize varieties. Isme Journal. 2013; 7(1), 37. pmid:22791236
- 9. Mougel C, Offre P, Ranjard L, Corberand T, Gamalero E, Robin C. Dynamic of the genetic structure of bacterial and fungal communities at different developmental stages of medicago truncatula gaertn. cv. jemalong line j5. New Phytologist. 2006; 170(1), 165–175. pmid:16539613.
- 10. Smalla K, Wieland G, Buchner A, Zock A, Parzy J, Kaiser S, et al. Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed. Applied & Environmental Microbiology. 2001; 67(10), 4742–4751. pmid:11571180
- 11. Fierer N, Leff JW, Adams BJ, Nielsen UN, Bates ST, Lauber CL et al. Cross-biome metagenomic analyses of soil microbial communities and their functional attributes.PNAS. 2012;109(52), 21390–21395. pmid:23236140
- 12. Liu J, Sui Y, Yu Z, Shi Y, Chu H, Jin J, et al. High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China. Soil Bio Biochem. 2014;70(2):113–122.
- 13. Caporaso JG, Bittinger K, Bushman FD, DeSantis TZ, Andersen GL, Knight R. PyNAST: a flexible tool for aligning sequences to a template alignment. Bioinformatics. 2010;26(2):266–267. pmid:19914921
- 14. Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol. 2007;73(16): 5261–5267. pmid:17586664
- 15. Johnston-Monje D, Lundberg DS, Lazarovits G, Reis VM, Raizada MN. Bacterial populations in juvenile maize rhizospheres originate from both seed and soil. Plant Soil. 2016; 405(1):337–355.
- 16. Buée M, Reich M, Murat C, Morin E, Nilsson RH. 454 Pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity. New Phytol. 2009;184(2):, 449–456. pmid:19703112
- 17. Whitman WB, Coleman DC, Wiebe WJ. Prokaryotes: the unseen majority. PNAS. 1998; 95(12): 6578–6583. pmid:9618454
- 18. Liebner S, Harder J, Wagner D. Bacterial diversity and community structure in polygonal tundra soils from Samoylov Island, Lena Delta, Siberia. Int Microbiol. 2008;11(11): 195–202. pmid:18843598
- 19. Goldfarb K.C., Karaoz U., Hanson C.A., Santee C.A., Bradford M.A., Treseder K.K., et al. Differential growth responses of soil bacterial taxa to carbon substrates of varying chemical recalcitrance. Front Microbiol. 2011;2(1):94. pmid:21833332
- 20. Adesemoye AO, Kloepper J W. Plant–microbes interactions in enhanced fertilizer-use efficiency. Appl Microbiol Biot. 2009;85(1):1–12. pmid:19707753
- 21. Adesemoye AO, Torber HA, Kloepper JW. (2009) Plant Growth-Promoting Rhizobacteria Allow Reduced Application Rates of Chemical Fertilizers. Microbiol Ecol. 2009;58(4): 921–929. pmid:19466478
- 22. Gupta Sood S. Chemotactic response of plant-growth-promoting bacteria towards roots of vesicular-arbuscular mycorrhizal tomato plants. Fems Microbiol Ecol. 2003;45(3):219–227. pmid:19719591
- 23. Singh BK, Millard P, Whiteley AS,Murrell JC. Unravelling rhizosphere-microbial interactions: opportunities and limitations. Trends Microbiol. 2004;12(8): 386–393. pmid:15276615