Bilateral carotid artery stenosis causes unexpected early changes in brain extracellular matrix and blood-brain barrier integrity in mice
Fig 2
Changes in the ECM are observed in multiple regions of the brain following BCAS.
A) Representative images of collagen IV staining (green) within the cortex, hippocampus and striatum of sham or BCAS-treated (7 and 14 days) mice. Scale bar = 100 um. Inset images are a magnified portion to show detail. Scale bar = 300 um. B) Quantification of collagen IV-positive pixels. N = 4–6 **p < 0.01 C) Representative images of alpha5 staining (red) within the cortex, hippocampus and striatum of sham or BCAS treated (7 and 14 days) mice. Scale bar = 100 um. Inset images are a magnified portion to show detail. Scale bar = 300 um. D) Quantification of alpha5-positive pixels. N = 4–6 *p < 0.05.
