Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system
Fig 1
Design of luciferase reporter T87 cells.
(A) A schematic diagram showing the vector constructs used in this study. 35S, sec, and T indicate the 35S promoter, secretory signal, and terminator polyadenylation signal, respectively. (B) Box plot of luminescence intensities for luciferase reporter Arabidopsis thaliana T87 cells on addition of furimazine or d-luciferin. Nano-Glo luciferase assay system and Dual-Glo luciferase assay system were used for NLuc and FLuc, respectively. The data are from three individual experiments with biological octuplicates; n = 24. Values indicate averages.
