Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system
Fig 3
Detection of reporter cell luminescence through a layer of T87 cells.
(A) Normalized emission spectra of NLuc (blue) and GeNL (green) reporter Arabidopsis thaliana T87 cells on addition of furimazine. The emission spectra of NLuc (blue dotted line) and GeNL (green dotted line) cells on the addition of coelenterazine h are also shown. The gray shade indicates the absorbance spectrum of wild-type T87 cells. The spectra were normalized to peak height for each reporter cell type in the range 400 to 700 nm. (B) Luminescence images of NLuc (blue) and GeNL (green) reporter T87 cells on addition of NLuc luciferin through a layer of wild-type T87 cells. (C) Transmittance rates of reporter cells covered with a layer of T87 cells. Transmittance rates were calculated as the transmitted luminescence intensity in the presence of a layer of wild-type T87 cells, divided by that in the absence of a layer. The values shown are the mean ± SE; n = 4. Student’s t test was performed. ***p < 0.001.
