The soluble VEGF receptor sFlt-1 contributes to endothelial dysfunction in IgA nephropathy
Fig 4
The apoptosis rate of HUVECs was measured using an Annexin V-APC apoptosis analysis kit.
The apoptosis of HUVECs was detected by flow cytometry. The figure showed a representative set of the dot-plot graph of flow cytometry analysis of the following groups: A: the indicated concentrations of sFlt-1-treated group, and B: statistical result of apoptosis rate of HUVECs in different groups. The apoptosis rate was up-regulated significantly as the stimulation concentrations of sFlt-1 increased (100 ng/ml vs. 0 ng/ml, 11.07 ± 1.57% vs. 3.47 ± 0.51%, p = 0.001; 1000 ng/ml vs. 0 ng/ml 12.77 ± 0.85% vs. 3.47 ± 0.51%, p < 0.001). There was no obvious difference between cells with 1000 ng/ml and 100 ng/ml (12.77 ± 0.85% vs. 11.07 ± 1.57%, p = 0.174). Results are the means ± standard deviation of three independent experiments.
