High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis
Fig 1
Agriculture (swine) exposure-related Organic Dust Extract (ODE) induced airway inflammation coupled with Collagen Induced Arthritis (CIA) model.
(A) Line graph depicts mean with SE bars of arthritis inflammatory score at respective time points from treatment groups. Statistical difference versus sham denoted as “a” (p<0.05); versus ODE denoted “b” (p<0.05); versus CIA denoted as “c” (p<0.05) as determined by two-way ANOVA. N = 8 mice/group from 3 independent studies. (B) Representative H&E-stained lung section from each treatment group at 10 X magnification with line scale (100 pixels). Representative images of lungs of CIA+ODE at 40x (parenchyma and peribronchiolar/perivascular regions), immunostained with CD3 (T cells) and CD45R (B cells) (C), and myeloperoxidase (MPO, neutrophils) and CD68 (macrophages) (D). Zoomed images highlight B-cell-T-cell interaction (C) and macrophages with neutrophils (D). CCR2+ inflammatory monocytes were increased with ODE and CIA treatment conditions in murine lungs. (E) Representative image of CCR2 expression (yellow) of lung tissue from each treatment group. (F) Bar graph depicts mean with standard error bars of CCR2 staining (N = 5/group). Statistical difference ***p<0.001 vs. saline/sham control and ###p<0.001 denoted by line between groups.
