High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis
Fig 5
Differences in transcript levels among monocyte-macrophages and dendritic cell (DC) populations.
(A) Heatmap shows the top 120/N upregulated genes for 7 macrophages/monocytes and DC clusters ranked by log2 fold-change, where N = total number of clusters. (B) Heatmap showing differences among top 5 genes with gene names of the macrophages/monocytes and DC populations. (C) Expression levels with violin plots among airspace macrophages (red, cluster 1), differentiating/recruited macrophages (brown, cluster 2), recruited macrophages (green, cluster 5), resident/interstitial macrophages (teal, cluster 11), proliferative airspace macrophages (light blue, cluster 14), inflammatory monocytes (lavender, cluster 12), dendritic cells (pink, cluster 10). The y-axis indicates normalized expression value, log2 (average UMI count + 1). (D) Distribution of respective cell populations by a representative gene in t-SNE plot.
