High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis
Fig 7
Treatment group-specific gene expression pattern demonstrated in isolated lung neutrophils.
Neutrophils were sorted from lung digests as live, singlets, CD45+, non-lymphocytes, Ly6C+ and Ly6G+. (A) Representative dot plots of Ly6C+ Ly6G+ neutrophils sorted from Sham, CIA, ODE and CIA+ODE treatment groups shown. (B) Heat map of fold-change of top 15 genes normalized to 20 housekeeping genes from each treatment group compared to Sham. (C) Bar graphs reflect the mean of normalized fold-change with standard error bars of representative genes from each treatment group Sham (red), CIA (green), ODE (teal), and CIA+ODE (blue). N = 3 (3 independent experiments with 2–3 mice pooled, 8 total mice). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
