A Major Role for the Plasmodium falciparum ApiAP2 Protein PfSIP2 in Chromosome End Biology
Figure 4
PfSIP2-N binds to SPE2 landmarks in subtelomeric regions of P. falciparum chromosomes in vivo.
(A) Genome-wide PfSIP2-N-HA occupancy as determined by high-density ChIP-on-chip. Schematic display (SignalMap) and localization of genomic regions bound by PfSIP2-N-HA in 3D7/SIP2-N-HA schizont stage parasites (blue lines) and the position of predicted SPE2 consensus sites (red lines) on all 14 parasite chromosomes. Genomic regions were considered occupied by PfSIP2-N-HA if the average of log2 ratios (ChIP over input) for all probes in a 1000bp window was higher than 1.4. Chromosome numbers are indicated on the left, chromosomal positions on top. Solid black lines indicate regions not represented on the microarray (see also Table S3). (B) Regional zoom-in of the PfSIP2-N-HA ChIP-on-chip profile and predicted SPE2 motifs at the left arm of chromosome 10. PfSIP2-N-HA-occupied regions (peaks) have been identified by the built-in algorithm of SignalMap as explained above. The locations of the upsB var gene and downstream rif genes are indicated below. Chromosomal coordinates are according to PlasmoDB version5.5 annotation (www.plasmodb.org).
