Variable Suites of Non-effector Genes Are Co-regulated in the Type III Secretion Virulence Regulon across the Pseudomonas syringae Phylogeny
Figure 2
Identification of a novel type III effector.
(A) Δ79avrRpt2 translocation assay. Four week old Col-0 and Col-0 rps2–101c (rps2) plants were hand inoculated with PtoDC300028E without plasmid or PtoDC300028E carrying either citrine (negative control), hopBA1 and its upstream region (positive control), IMG/ER Porcuarted_02784, 04644, 04640, 03530, 02145, and 04371 sequence and upstream sequence into Δ79avrRpt2 -fusion vector pJC532. Plants were scored and pictures were taken 24 h after inoculation for hypersensitive response (HR). (B) Western blotting analysis. PtoDC300028E without plasmid (negative control), PtoDC300028E carrying IMG/ER Porcurated_02784, 04644, 04640, 03530, 02145, and 04371 sequence and upstream region into HA-fusion vector pJC531 were grown on MM media for 5 hours. Culture aliquots were subjected to western blot using anti-HA antibody. CBB, Coomassie brilliant blue.
