Variable Suites of Non-effector Genes Are Co-regulated in the Type III Secretion Virulence Regulon across the Pseudomonas syringae Phylogeny
Figure 4
hrp-box mutations associated with differential HrpL-dependent up-regulation of PSPTO_2105, and 2130 orthologs.
(A) PSPTO_2105 and PSPTO_2130 hrp-box sequence variation across P. syringae strains as presented in [3]. In blue, canonical hrp-box nucleotides. In red, nucleotides of divergent hrp-box sequences. (B) HrpL-dependent expression of PSPTO_2130 orthologs across P. syringae strains. qRT-PCR analysis was performed on RNA samples derived from isogenic strains expressing, or lacking, an arabinose-inducible hrpL gene. Expression was normalized to gap-1. Relative expression: each EV sample was set to 1 and HrpL samples normalized to the corresponding EV samples. Error bars represent SD. (C) HrpL-dependent expression of PSPTO_2105 orthologs across P. syringae strains, as above. Each experiment was repeated twice.
