The Epstein-Barr Virus-Encoded MicroRNA MiR-BART9 Promotes Tumor Metastasis by Targeting E-Cadherin in Nasopharyngeal Carcinoma
Figure 7
miR-BART9 induces β-catenin translocation and a mesenchymal-like morphology in EBV-negative NPC cells.
(A) Representative immunofluorescence staining of β-catenin and DAPI staining to detect the nucleus in BM1 cells expressing miR-BART9 or LacZ. Scale bar = 20 µm. (B) Phase contrast images of BM1 and TW04 cells infected with an miR-BART9-expressing vector (BART9) or control vector (LacZ). Cells were plated in 60-mm dishes at the same density. Images were acquired 9 days after plating. (C) Phase contrast images of BM1 and TW04 cells infected with an miR-BART9-expressing vector (BART9) or control vector (LacZ). Cells were seeded on 60-mm dishes at the same density. Images were acquired 2 days after seeding. Scale bar = 10 µm. (D) DAPI (nucleus) and FITC-conjugated wheat germ agglutinin (WGA) staining in BM1 and TW04 cells expressing the miR-BART9 or control vector (LacZ). Arrowheads indicate filopodia structures. Scale bar = 20 µm. (E) DAPI (nucleus) and Alexa Fluor 594-conjugated phalloidin (F-actin) staining in BM1 and TW04 cells expressing the miR-BART9 or control vector (LacZ). Arrowheads indicate stress fibers. Scale bar = 20 µm.
