Non-human Primate Schlafen11 Inhibits Production of Both Host and Viral Proteins
Fig 3
Schlafen11 reduces protein production from non-viral transcripts.
(A) Schlafen11 or Chlor (negative control) expressing plasmids were cotransfected into 293T cells along with plasmids expressing either a V5-tagged GFP or myc-tagged eGFP. Total protein was harvested 48 hours post transfection and probed for the indicated proteins. The blot is representative of 3 independent experiments. (B) Either GFP or eGFP (untagged) expressing plasmids were cotransfected along with plasmids encoding Schlafen11 as in (A). 48 hours post transfection, flow cytometric analysis was performed to measure the mean fluorescence intensity (MFI) of the GFP signal. All values reported are relative the the Chlor control. Error bars are standard error from 3 independent experiments.
