Non-human Primate Schlafen11 Inhibits Production of Both Host and Viral Proteins
Fig 4
Schlafen11 inhibits production of human proteins.
(A) Codon adaptation index (CAI) is plotted for all human genes, and for select other genes (viral and GFP/eGFP) used in this study. Human genes analyzed in this study are indicated in red, viral genes in purple. (B,C,D) Plasmids encoding the indicated Schlafen11-V5 proteins, or Chlor, were cotransfected into 293T cells (B) with or without a Vinculin-V5 expressing plasmid, (C) with a GAPDH-V5 expressing plasmid, or (D) with an Actin-V5 expressing plasmid. Cell lysates were subject to immunoblotting as indicated. Blots are representative from two independent experiments. (E) Quantification of the V5-tagged proteins detected in panels B-D. All bands are normalized to the quantification of endogenous GAPDH.
