Non-human Primate Schlafen11 Inhibits Production of Both Host and Viral Proteins
Fig 5
Schlafen11 is expressed in various human tissues and is active at physiologically-relevant levels.
(A) 293T cells were cotransfected with viral packaging plasmids required to create VSV G-pseudotyped MLV along with increasing amounts of pcDNA6.2 encoding the indicated Schlafen11 or Chlor. Pseudotyped MLV produced was measured on 293T cells and the relative amount of virus production (standardized to Chlor) was determined. (B) Identical to (A), except performed in HUT78 cells (a T-cell line). Only the results from 1000ng is shown as other experiments did not yield replicable results. (C) Identical to (A), except performed in Chinese Hamster Ovary (CHO) cells. (D) Quantitative PCR was used to measure SLFN11 expression levels in cDNA from transfection conditions in panels A-C, and from a set of human tissues. Error bars represent the standard error of three independent replicate experiments. (E) SLFN11 was amplified by non-quantitative PCR from a cDNA panel representing the human tissues indicated.
