Vibrio cholerae ensures function of host proteins required for virulence through consumption of luminal methionine sulfoxide
Fig 2
Mutation of the V. cholerae glycine cleavage system alters the extracellular environment in LB broth, Drosophila intestine, and the rabbit intestine.
LC-MS/MS based metabolomic analysis of wild-type V. cholerae (WT) or a ΔgcvT mutant in (A and B) LB culture supernatants, (C) infected Drosophila intestines, and (D) cecal fluid of infected infant rabbits. Only metabolites that were significantly different under the two conditions are shown (p<0.05). (E) Levels of MetO and methionine (Met) in LB broth alone, in the intestines of flies fed LB broth alone (LB), LB broth inoculated with wild-type V. cholerae (WT) or LB broth inoculated with a V. cholerae ΔgcvT mutant, and in the cecal fluid of infant rabbits inoculated with wild-type V. cholerae or a V. cholerae ΔgcvT mutant. (F) Compounds identified in cecal fluid metabolomics are shown in green along with their relationship to the methionine cycle. For pooled data, the mean and SD are shown.
