Vibrio cholerae ensures function of host proteins required for virulence through consumption of luminal methionine sulfoxide
Fig 6
As compared with infection with wild-type V. cholerae, Drosophila infection with a V. cholerae ΔgcvT mutant results in an increase in oxidation of protein-associated methionine within the intestine.
Western blot analysis of proteins in the intestines of Drosophila fed (A) conventional LB broth (LB) and fly food (Food) or (B) a wild-type V. cholerae (WT) and a ΔgcvT mutant. An antibody recognizing oxidized methionine (anti-MetO) was used. BSA-MetO protein was used as a positive control (CTL), and tubulin was used as a loading control (Tub). (C-E) Densitometry analysis of indicated bands and entire lanes (Total) for Western blots shown in (A) and (B). Relative density represents the density measurement for the individual band or lane normalized to the density measurement of the relevant tubulin loading control. Experimental replicates were performed with similar trends noted.
