Profiling of immune related genes silenced in EBV-positive gastric carcinoma identified novel restriction factors of human gammaherpesviruses
Fig 4
Down-regulation of MT1 and HOXA genes in EBV-positive GC and Burkitt’s lymphoma cell lines.
(A, B) AGS-BX and AGS gastric cancer cells were subjected to RT-qPCR assays to measure the expression of MT1 (A) and HOXA (B) genes. Relative expression in AGS-BX (Red) of each gene was normalized with respect to parental AGS cells (Blue). (C, D) Akata-BX and Akata Burkitt's lymphoma cells were assayed similarly as (A, B) to measure the expression of MT1 (C) and HOXA (D) genes. Relative expression in Akata-BX (Red) of each gene was normalized with respect to parental Akata cells (Blue). (E, F). AGS-BX cells were treated with DNA methylation inhibitors (5-azacytine, 5-aza-2-deoxycytine), or vehicle control, followed by the RT-qPCR assays to measure the expression of selected MT1 (E) and HOXA (F) genes. Results are based on n = 3 independent repeats and presented as mean ± SEM. (* p<0.05; ** p<0.01; *** p<0.001, two-tailed paired Student t-test).
